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Hydrogen Peroxide Test Kits

Visual & Titrimetric Kits

Range MDL Method Type Kit Cat. No. Refill
0.0 - 0.5 ppm 0.025 ppm DPD CHEMets K-5502 R-5502
0.0 - 0.8 & 1 - 10 ppm 0.05 ppm Ferric Thiocyanate CHEMets K-5510 R-5510
0 - 25 & 30 - 300 ppm 5 ppm Ferric Thiocyanate VACUettes K-5510D R-5510D
0 - 50 & 60 - 600 ppm 10 ppm Ferric Thiocyanate VACUettes K-5510A R-5510A
0 - 100 & 120 - 1,200 ppm 20 ppm Ferric Thiocyanate VACUettes K-5510B R-5510B
0 - 1,000 & 1,200 - 12,000 ppm 200 ppm Ferric Thiocyanate VACUettes K-5510C R-5510C
0.1 - 1.0 % (2 - 20 % w/dilution)* 0.10% Ceric Sulphate Titrant / Ferroin Titrets K-5530

Instrumental Kits

Range Method Type Kit Cat. No.
0 - 6.00 ppm Ferric Thiocyanate Vacu-vials K-5543
0 - 6.00 ppm Ferric Thiocyanate SAM Photometer + Vacu-vials I-2016

Note: Click on the ‘Kit Cat. No.’ to view the test instructions. Click on the ‘Range’ value for Vacu-vials to view accuracy chart.

The CHEMetrics test kits for the determination of Hydrogen Peroxide in aqueous solutions are based on patented Self-Filling Reagent Ampoule technology, and employ the Ferric Thiocyanate, DPD and Ceric Sulphate methods, delivering sensitivity and accuracy within two minutes or less. Premixed. Premeasured. Precise. Each kit contains 30 tests. Suitable for food and beverage industry applications as well as industrial effluent testing.

The Ferric Thiocyanate Method

The ferric thiocyanate method consists of ammonium thiocyanate and ferrous iron in an acidic solution. Hydrogen peroxide oxidises ferrous to ferric iron, resulting in the formation of a red thiocyanate complex. Chlorine does not interfere with this method, but Ferric iron does. Results are expressed as ppm (mg/l) H2O2.

D. F. Boltz and J. A. Howell, eds., Colorimetric Determination of Nonmetals, 2nd. ed., Vol. 8, p. 304 (1978).

The DPD Method

With the DPD Method, hydrogen peroxide in the sample reacts with DPD (N, N-diethyl-p-phenylenediamine) in the presence of potassium iodide and ammonium molybdate to form a pink colour in the ampoule. Results are expressed as ppm (mg/l) H2O2.

USEPA Methods for Chemical Analysis of Waters and Wastes, Method 330.5 (1983).
APHA Standard Methods, 21st ed., Method 4500-Cl G (2005).
D.F. Boltz and J.A. Howell, eds., Colorimetric Determination of Nonmetals 2nd ed., Vol. 8, p. 303 (1978).

The Ceric Sulphate Titrimetric Method

CHEMetrics has developed a patented reverse titrimetric method using ceric sulphate as the titrant and ferroin as the end point indicator. A colour change from green to orange signals the end of the titration. Results are expressed as percent (%) H2O2. The test range can be also be modified by performing a sample dilution. Details are provided in the kit instructions for testing samples in the ranges of 0.01 - 0.1% through 2- 20%.

* Up to 20% with dilution

Developed by CHEMetrics.


Hydrogen peroxide is a strong oxidising agent with a wide variety of uses. Applications include the treating of industrial effluents and domestic waste and serving as a disinfectant in aseptic packaging. For the food and beverage industry, CHEMetrics Hydrogen Peroxide CHEMets® and Vacu-vials® products are used extensively to monitor sterilisation solution residuals in Extended Shelf Life (ESL) and aseptic packaging applications. The product cartons are sprayed with hydrogen peroxide to pre-sterilise them, and then heated or pressed to remove the hydrogen peroxide. A hydrogen peroxide solution is also used to clean and sterilise manufacturing equipment used in the aseptic packaging processing line.

Comparison with EMD Millipore's MQuant Test Strips

This CHEMetrics Report examines statistical data from 2018 for 2 CHEMetrics Hydrogen Peroxide Test Kits, CHEMets® K-5510 Visual Test Kit and K-5543 Vacu-vials® Photometric Test Kit, and EMD Millipore's MQuant™ Test Strips (Part No. 110011). The K-5543 was measured using 2 CHEMetrics photometers, I-2016 Dedicated Photometer (SAM) and V-2000 Multi-Analyte Phometer. 4 H2O2 standard concentrations were measured, 0, 0.15, 0.25 and 0.50 ppm. Whilst the CHEMetrics kits were able to distinguish concentrations in 0.05 ppm increments and an accuracy of ± 0.02 ppm respectively, MQuant™ Test Strips had only 2 pale colour intervals between 0 & 0.5 ppm, and whilst it was possible to distinguish between 0, 0.25 and 0.5 ppm, colour development was barely perceptible at 0.15ppm.

CHEMetrics® Hydrogen Peroxide Self-filling Ampoules vs Test Strips

Standard Solution

CHEMetrics® offers a standard solution for hydrogen peroxide, catalogue no. A-5505, for the preparation of 0.5 ppm standard, offers the analyst an easy-to-use, NIST Traceable standard to confirm the accuracy and reliability of hydrogen peroxide measurements for test kits K-5502, K-5510 and K-5543.

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