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Cyanide (Free) Test Kits

Visual Kit

Range MDL Method Type Kit Cat. No. Refill
0.00 - 0.10 & 0.1 - 1.0 ppm 0.005 ppm Isonicotinic-Barbituric Acid CHEMets K-3810 R-3810

Instrumental Kit

Range Method Type Kit Cat. No.
0 - 0.400 ppm Isonicotinic-Barbituric Acid Vacu-vials K-3803

Note: Click on the ‘Kit Cat. No.’ to view the test instructions. Click on the ‘Range’ value for Vacu-vials to view accuracy chart.

CHEMetrics offers test kits employing the well-known Isonicotinic-Barbituric Acid reagent to deliver sensitivity and accuracy within two minutes or less. Based on CHEMetrics patented Self-Filling Reagent Ampoule technology. Premixed. Premeasured. Precise. Each kit contains 30 tests.

The Isonicotinic-Barbituric Acid Method

In the Cyanide CHEMets® and Vacu-vials® Kit, chlorine is added to a sample that has been buffered to pH 6. The resulting cyanogen chloride reacts with isonicotinic and barbituric acids to form a blue colour. Results are expressed as ppm (mg/l) CN.

This chemistry provides two advantages over the more commonly used Pyridine methods:

S. Nagashima, Spectrophotometric Determination of Cyanide with Isonicotinic Acid and Barbituric Acid, International Journal of Environ. Anal. Chem., 1981, Vol. 10, pp. 99-106.


Cyanide is used in many chemical and refining processes. It is found in effluent from electroplating and metal cleaning operations, coke ovens, steel manufacturing facilities, and gas scrubbers. Low levels may occur naturally in groundwaters after it has passed through mineral deposits and rock strata. Although cyanide can be safely removed by alkaline chlorination, its acute toxicity to aquatic life necessitates routine monitoring of effluents. The Directive Prescribed Concentration or Value (PCV) for free cyanide in drinking water in the UK and Ireland is 50 µg/l (ppb).

CHEMetrics' cyanide test kits are applicable to the monitoring of effluents and surface water supplies. It is recommended, however, that the sample be distilled and hydrogen sulphide be removed prior to analysis.

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